• [Mechanisms of evolution: Grades 9-12] Speciation is the splitting of one ancestral lineage into two or more descendant lineages.
• [Mechanisms of evolution: Grades 9-12] Speciation is often the result of geographic isolation.
• [Mechanisms of evolution: Grades 9-12] Speciation requires reproductive isolation.
• [Mechanisms of evolution: Grades 13-16] Speciation is the splitting of one ancestral lineage into two or more descendant lineages.
• [Mechanisms of evolution: Grades 13-16] Speciation is often the result of geographic isolation.
• [Mechanisms of evolution: Grades 13-16] Speciation requires reproductive isolation.
• [Nature of science: Grades 9-12] Scientific knowledge is open to question and revision as we come up with new ideas and discover new evidence. (P4, P6, NOS3)
• [Nature of science: Grades 9-12] A hallmark of science is exposing ideas to testing. (P3, P4, P6, P7)
• [Nature of science: Grades 9-12] Scientists test their ideas using multiple lines of evidence. (P6, NOS2)
• [Nature of science: Grades 9-12] Scientists use multiple research methods (experiments, observational research, comparative research, and modeling) to collect data. (P2, P3, P4, NOS1)
• [Nature of science: Grades 9-12] Scientists can test ideas about events and processes long past, very distant, and not directly observable.
• [Nature of science: Grades 9-12] The real process of science is complex, iterative, and can take many different paths.
• [Nature of science: Grades 9-12] Science is a human endeavor. (NOS7)
• [Nature of science: Grades 13-16] Scientific knowledge is open to question and revision as we come up with new ideas and discover new evidence.
• [Nature of science: Grades 13-16] A hallmark of science is exposing ideas to testing.
• [Nature of science: Grades 13-16] Scientists test their ideas using multiple lines of evidence.
• [Nature of science: Grades 13-16] Scientists use multiple research methods (experiments, observational research, comparative research, and modeling) to collect data.
• [Nature of science: Grades 13-16] The real process of science is complex, iterative, and can take many different paths.
• [Nature of science: Grades 13-16] Science is a human endeavor.
• [Studying evolution: Grades 9-12] Scientists use the similarity of DNA nucleotide sequences to infer the relatedness of taxa. (LS4.A)
• [Studying evolution: Grades 9-12] Scientists use anatomical evidence to infer the relatedness of taxa. (LS4.A)
• [Studying evolution: Grades 9-12] Scientists use the geographic distribution of fossils and living things to learn about the history of life.
• [Studying evolution: Grades 9-12] Scientists use experimental evidence to study evolutionary processes.
• [Studying evolution: Grades 13-16] Scientists use multiple lines of evidence (including morphological, developmental, and molecular evidence) to infer the relatedness of taxa.
• [Studying evolution: Grades 13-16] Scientists use the geographic distribution of fossils and living things to learn about the history of life.
• [Studying evolution: Grades 13-16] Scientists use experimental evidence to study evolutionary processes.

• Disciplinary Core Idea LS4.A: Evidence of Common Ancestry and Diversity
• NOS Matrix understanding category 1. Scientific investigations use a variety of methods.
• NOS Matrix understanding category 2. Scientific knowledge is based on empirical evidence.
• NOS Matrix understanding category 3. Scientific knowledge is open to revision in light of new evidence.
• Science and Engineering Practice 2. Developing and using models
• Science and Engineering Practice 3. Planning and carrying out investigations
• Science and Engineering Practice 4. Analyzing and interpreting data
• Science and Engineering Practice 6. Constructing explanations and designing solutions
• Science and Engineering Practice 7. Engaging in argument from evidence

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• Evidence for Evolution
• Speciation